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Table 1 Effect of different detergents on the release of PhaC1, PhaC2 and other proteins from PHA granules.

From: Overexpression and characterization of medium-chain-length polyhydroxyalkanoate granule bound polymerases from Pseudomonas putida GPo1

Extractant (w/v)a

PhaC1

PhaC2

PhaF

PhaI

 

Proteins released from PHA granules (%)b

Tris-HCl pH 8 (100 mM)

n.dc

n.d

n.d

n.d

Phospholipids (0.5%)

n.d

n.d

n.d

n.d

Hecameg (0.5%)

50%

n.d

n.d

10%

Triton X-100 (0.5%)

100%

0-20%

10%

10%

CHAPS (0.5%)

100%

0-20%

n.d

25%

Igepal-CA630 (0.5%)

100%

0-20%

n.d

25%

Rhamnolipids (0.1%)

100%

100%

75%

75%

  1. a) Detergents were dissolved in 100 mM Tris-HCl (pH 8).
  2. b) Percentages of protein release were determined by comparing granule pellet fractions and supernatant fractions on SDS-PAA gels.
  3. c) Not detected (< 2% of total granule bound protein).