Figure 2
Substrate specificity of granule-bound PhaC1 and PhaC2. Assay conditions: 100 mM Tris-HCl pH 8, 1 mg/ml BSA, 0.5 mM MgCl2, 0.2 μg/ml granule bound PhaC1 or 2 μg/ml granule bound PhaC2 with 0.25 mM of one of the following acyl-CoA substrates: R/S- 3-hydroxybutyryl-CoA (C4), R- 3-hydroxyhexanoyl-CoA (C6), R- 3-hydroxyoctanoyl-CoA (C8), R- 3-hydroxydecanoyl-CoA (C10) and R- 3-hydroxydodecanoyl-CoA (C12). Initial activity was measured spectrophotometrically (A412) by following release of CoA using DTNB.