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Figure 1 | Microbial Cell Factories

Figure 1

From: Overexpression and characterization of medium-chain-length polyhydroxyalkanoate granule bound polymerases from Pseudomonas putida GPo1

Figure 1

SDS-PAGE of different fractions following purification of PhaC1 (A) and PhaC2 (B) and comparison of native PhaC1 and PhaC2 (C). Lanes: 1, molecular mass standards (kDa); 2, crude extract of P. putida GPp104 carrying pGEc405 (A) or pGEc404 (B); 3, isolated PHA granules; 4, released proteins using 0.5% (v/v) Triton X-100 (A) or 0.1% (w/v) rhamnolipids (B); 5, purified PhaC1 (A) and PhaC2 (B) after Source15Q chromatography; 6, purified native PhaC1 (C); 7, purified native PhaC2 (C).

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Microbial Cell Factories

ISSN: 1475-2859

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