Figure 3

Growth and GFP expression during fed batch fermentations with either one or four impellers (all else equal). (A) Biomass, measured as optical density (OD, A₆₀₀), and glucose concentration (g/L), (B) Green fluorescent protein expression (RFU) and dissolved oxygen concentration (% air saturation) and (C) specific GFP expression (RFU/A₆₀₀). Dashed vertical line at 21 hours shows where batch phase ended and feeding began. Error bars represent standard deviation, drawn in only one direction for clarity.