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Table 2 Kinetic parameters for the purified mannan endo-1,4-β-mannosidasea at 70°C

From: Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-β-mannosidase from Aspergillus niger BK01

Substrate

Vmax(U mg-1)

Km(mg mL-1)

kcat(s-1)

kcat/Km(mg-1 s-1 mL)

Glucomannan

243 ± 15

0.6 ± 0.3

215

358

Locust bean gum

373 ± 19

2.0 ± 0.4

330

165

Galactomannan

330 ± 9

2.2 ± 0.2

292

133

Mannan

167 ± 5

1.5 ± 0.2

148

98.4

Guar gum

398 ± 48

7.7 ± 1.8

352

45.7

  1. a Enzymatic reactions were carried out for 5 min at 70°C in 0.07 M citrate phosphate buffer (pH 4.5).
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Microbial Cell Factories

ISSN: 1475-2859

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