Figure 3From: Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-β-mannosidase from Aspergillus niger BK01Deglycosylation of recombinant A. niger mannan endo- β-1,4-mannosidase. SDS-PAGE analysis of the recombinant mannan endo-1,4-β-mannosidase after deglycosylation with endoglycosidase H. Lane M: molecular mass standard protein; Lane 1: purified β-mannanase; Lane 1#: diluted purified β-mannanase; Lane 2: deglycosylated β-mannanase after endoH treatment; Lane 2#: diluted deglycosylated β-mannanase; Lane 3: endoglycosidase H.Back to article page