Figure 2From: Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-β-mannosidase from Aspergillus niger BK01SDS-PAGE and zymogram analysis of recombinant A. niger mannan endo- β-1,4-mannosidase. SDS-PAGE (A) and zymogram (B) of secreted proteins from recombinant Pichia pastoris expressing the A. niger β -mannanase are shown. Lane M, molecular mass standard protein; Lane 1-6, culture supernatant after induction for 6, 24, 36, 48, 72 and 96 h, respectively; Lane C, control of P. pastoris X33 harbouring the empty pPICZαA vector after induction for 96 h. Twelve μL of culture supernatant was loaded onto each lanes.Back to article page