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Table 2 Export efficiency of preLCI

From: Engineering an efficient secretion of leech carboxypeptidase inhibitor in Escherichia coli

 

pOmpALCIN

pOmpALCIO

pDsbALCIN

pDsbALCIO

pMalEssLCIN

pMalEssLCIO

R1

0.4

0.1

1.3

4.8

1.0

1.8

R2

0.2

0.01

1.1

5.7

0.9

1.5

  1. The relative amount of precursor (p) and mature (m) LCI present in each gel shown in figure 3 was assessed by densitometric scanning and the ratio R (m/p) was determined from whole cell lysates (R1), and from cytoplasmic and periplasmic fractions (R2).
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Microbial Cell Factories

ISSN: 1475-2859

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