Engineering an efficient secretion of leech carboxypeptidase inhibitor in Escherichia coli

Table 1 Distribution of native LCI between cells and culture medium

	pOmpALCI_N	pOmpALCI_O	pDsbALCI_N	pDsbALCI_O	pMalEssLCI_N	pMalEssLCI_O
Whole cell lysates	53	33.5	147.5	154.5	104	125.5
Culture supernatants	nd	nd	nd	12.5	nd	4

The level of LCI (mg. l^-1) in these fractions was assayed by a spectrophotometric assay using bovine carboxypeptidase A, and normalized to protein content of whole cell lysates determined by the Bradford procedure using bovine serum albumin as a standard (nd, not detectable).

ISSN: 1475-2859