Figure 3
From: Engineering an efficient secretion of leech carboxypeptidase inhibitor in Escherichia coli
Steady-state level and cellular location of LCI. Whole cell lysates (A), cytoplasmic fractions (B), and periplasmic fractions (C) were separated by SDS-PAGE and stained by Coomassie blue. Lanes 1, pLCB; lanes 2, pOmpAssLCIN; lanes 3, pOmpAssLCIO; lanes 4, pMalEssLCIN; lanes 5, pMalEssLCIO; lanes 6, pDsbAssLCIN; lanes 7, pDsbALCIO. The position of precursor (p) and mature (m) LCI is indicated by an arrow.