Figure 1From: Engineering an efficient secretion of leech carboxypeptidase inhibitor in Escherichia coliCodon optimization of the mature LCI sequence. The natural and E. coli optimized nucleotide sequences coding the mature LCI, indicated in blue, are aligned. Codons occurring at a frequency below 8‰ in E. coli are underlined and codon changes are indicated in red. The codon adaptation index or CAI [28] shifts from 0.209 for the natural sequence to 0.622 for the optimized sequence.Back to article page