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Table 5 Effects of endoglycosidase-H treatment on Kpkt killer activity.

From: Tetrapisispora phaffii killer toxin is a highly specific β-glucanase that disrupts the integrity of the yeast cell wall

Conditions

After 24 h incubation

(CFU × 105 ml-1)

After 48 h incubation

(CFU × 105 ml-1)

Without Kpkt

17.0 ± 1.27

56.0 ± 8.48

With Kpkt

0.5 ± 0.14

0.03 ± 0.016

With Kpkt + Endo H

6.4 ± 0.42

6.7 ± 0.56

With Kpkt and without Endo H

0.29 ± 0.098

0.43 ± 0.013

  1. The initial inoculum of sensitive cells was 2 × 105 cell ml-1. Data are means ± SD of three experiments.
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Microbial Cell Factories

ISSN: 1475-2859

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