Tetrapisispora phaffii killer toxin is a highly specific β-glucanase that disrupts the integrity of the yeast cell wall

Table 3 Kpkt activity in the presence and absence of sorbitol as an osmotic stabilizer

Medium	% Dead cells^a
	Thoma-Zeiss counting chamber	Viable cell counts After 48 h
Whole sensitive cells + Kpkt in 0.8 molar sorbitol	5.4 ± 1.0	4.0 ± 0.8
Whole sensitive cells + Kpkt	55.4 ± 5.2	93.9 ± 3.0

^a The initial inoculum (10⁶ cell ml^-1) was considered as 100%. Data are means ± SD of three experiments.

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