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Table 1 Effects of the β-glucanase inhibitor castanospermine on Kpkt killer activity.

From: Tetrapisispora phaffii killer toxin is a highly specific β-glucanase that disrupts the integrity of the yeast cell wall

Purified Kpkt

Enzymatic activity

(g l-1 glucose)

Killer activity

Ø halo in well test

(mm)

Viable cell counts

CFU ml-1

(sensitive cells)

No castanospermine

0.032

11.0

0.5 × 102

25 μM castanospermine

0.0001

0.0

1.5 × 105

  1. The Kpkt treatment was carried out with 25 μg toxin for 24 h at 25°C. The initial inoculum of the sensitive strain was 105 cell ml-1. Growth of the sensitive cells was measured through viable plate counts.
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Microbial Cell Factories

ISSN: 1475-2859

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