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Table 4 Enzyme activities in the control and Δpta mutant strain.

From: An insight into the role of phosphotransacetylase (pta) and the acetate/acetyl-CoA node in Escherichia coli

  Glucose aerobic
batch cultures
   Acetate aerobic
batch cultures
   Glucose anaerobic
batch cultures
  Exponential phase Stationary phase Exponential phase Stationary phase Exponential phase
  BW25113 pta- BW25113 pta- BW25113 pta- BW25113 pta- BW25113 pta-
MDH 132.6 ± 3.1 124.0 ± 25.2 66.7 ± 17.7 74.3 ± 0.4 41.7+10.0 53.9 ± 6.8 57.3 ± 2.2 62.2 ± 5.6 71.6 ± 3.6 60.3 ± 8.7
ICDH 298.3 ± 3.4 432.6 ± 16.7 351.5 ± 51.0 502.2 ± 21.6 376.2 ± 14.4 243.7 ± 33.0 318.5 ± 8.6 158.4 ± 5.5 129.3 ± 4.0 136.6 ± 18.8
ICL 18.4 ± 0.8 15.1 ± 2.3 15.2 ± 3.7 9.6 ± 0.8 276.2 ± 31.9 187.2 ± 4.4 242.7 ± 27.4 211.4 ± 23.7 12.1 ± 0.5 5.9 ± 0.9
ACS n.a. n.a. 18.0 ± 1.4 5.6 ± 0.1 28.4 ± 9.8 0.3 ± 0.5 10.5 ± 2.3 4.6 ± 0.2 101.6 ± 1.9 10.0 ± 0.1
PTA 897.7 ± 10.3 n.a. 1716.7 ± 168 n.a. 1140.0 ± 39.1 n.a. 2441.8 ± 121.8 n.a. 1691.8 ± 231.2 n.a.
POX 240.6 ± 1.65 164.6 ± 16.6 231.5 ± 45.9 261.0 ± 14.1 323.6 ± 34.5 431.3 ± 21.7 441.05 ± 24.3 481.4 ± 43.8 251.9 ± 25.1 205.1 ± 8.2
PDH 249.3 ± 3.9 505.9 ± 61.8 293.6 ± 39.4 605.8 ± 106.3 141.3 ± 39.6 96.7 ± 15.4 204.5 ± 5.6 126.3 ± 25.8 211.1 ± 29.0 273.0 ± 16.2
PYK 184.6 ± 51.9 303.4 ± 59.9 273.9 ± 65.3 377.5 ± 17.4 189.6 ± 21.9 176.8 ± 15.9 257.2 ± 8.7 315.0 ± 14.2 461.9 ± 61.9 512.1 ± 17.6
ZWF 124.1 ± 23.1 140.1 ± 7.8 117.4 ± 33.0 173.9 ± 24.3 68.5 ± 14.3 100.2 ± 23.1 82.0 ± 22.5 128.6 ± 4.4 232.2 ± 30.6 129.0 ± 10.7
ICDH/ICL 16.2 ± 0.8 28.7 ± 4.5 23.1 ± 3.6 52.6 ± 4.8 1.4 ± 0.2 1.3 ± 0.2 1.3 ± 0.2 0.7 ± 0.1 10.7 ± 0.5 23.2 ± 4.7
PTA/ACS n.a. n.a. 95.4 ± 4.5 n.a. 40.1 ± 13.9 n.a. 231.5 ± 51.2 n.a. 16.6 ± 2.3 n.a.
  1. Samples were taken at mid-exponential phase and at the beginning of the stationary phase. All enzyme activities are expressed as mU·mg-1 protein. Experimental conditions are expressed in the Materials and Methods section. (n.a.- not applicable; n.d.- not detected).