Table 4 Enzyme activities in the control and Δpta mutant strain.
Glucose aerobic batch cultures | Acetate aerobic batch cultures | Glucose anaerobic batch cultures | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Exponential phase | Stationary phase | Exponential phase | Stationary phase | Exponential phase | ||||||
BW25113 | pta- | BW25113 | pta- | BW25113 | pta- | BW25113 | pta- | BW25113 | pta- | |
MDH | 132.6 ± 3.1 | 124.0 ± 25.2 | 66.7 ± 17.7 | 74.3 ± 0.4 | 41.7+10.0 | 53.9 ± 6.8 | 57.3 ± 2.2 | 62.2 ± 5.6 | 71.6 ± 3.6 | 60.3 ± 8.7 |
ICDH | 298.3 ± 3.4 | 432.6 ± 16.7 | 351.5 ± 51.0 | 502.2 ± 21.6 | 376.2 ± 14.4 | 243.7 ± 33.0 | 318.5 ± 8.6 | 158.4 ± 5.5 | 129.3 ± 4.0 | 136.6 ± 18.8 |
ICL | 18.4 ± 0.8 | 15.1 ± 2.3 | 15.2 ± 3.7 | 9.6 ± 0.8 | 276.2 ± 31.9 | 187.2 ± 4.4 | 242.7 ± 27.4 | 211.4 ± 23.7 | 12.1 ± 0.5 | 5.9 ± 0.9 |
ACS | n.a. | n.a. | 18.0 ± 1.4 | 5.6 ± 0.1 | 28.4 ± 9.8 | 0.3 ± 0.5 | 10.5 ± 2.3 | 4.6 ± 0.2 | 101.6 ± 1.9 | 10.0 ± 0.1 |
PTA | 897.7 ± 10.3 | n.a. | 1716.7 ± 168 | n.a. | 1140.0 ± 39.1 | n.a. | 2441.8 ± 121.8 | n.a. | 1691.8 ± 231.2 | n.a. |
POX | 240.6 ± 1.65 | 164.6 ± 16.6 | 231.5 ± 45.9 | 261.0 ± 14.1 | 323.6 ± 34.5 | 431.3 ± 21.7 | 441.05 ± 24.3 | 481.4 ± 43.8 | 251.9 ± 25.1 | 205.1 ± 8.2 |
PDH | 249.3 ± 3.9 | 505.9 ± 61.8 | 293.6 ± 39.4 | 605.8 ± 106.3 | 141.3 ± 39.6 | 96.7 ± 15.4 | 204.5 ± 5.6 | 126.3 ± 25.8 | 211.1 ± 29.0 | 273.0 ± 16.2 |
PYK | 184.6 ± 51.9 | 303.4 ± 59.9 | 273.9 ± 65.3 | 377.5 ± 17.4 | 189.6 ± 21.9 | 176.8 ± 15.9 | 257.2 ± 8.7 | 315.0 ± 14.2 | 461.9 ± 61.9 | 512.1 ± 17.6 |
ZWF | 124.1 ± 23.1 | 140.1 ± 7.8 | 117.4 ± 33.0 | 173.9 ± 24.3 | 68.5 ± 14.3 | 100.2 ± 23.1 | 82.0 ± 22.5 | 128.6 ± 4.4 | 232.2 ± 30.6 | 129.0 ± 10.7 |
ICDH/ICL | 16.2 ± 0.8 | 28.7 ± 4.5 | 23.1 ± 3.6 | 52.6 ± 4.8 | 1.4 ± 0.2 | 1.3 ± 0.2 | 1.3 ± 0.2 | 0.7 ± 0.1 | 10.7 ± 0.5 | 23.2 ± 4.7 |
PTA/ACS | n.a. | n.a. | 95.4 ± 4.5 | n.a. | 40.1 ± 13.9 | n.a. | 231.5 ± 51.2 | n.a. | 16.6 ± 2.3 | n.a. |