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Table 2 Investigated E. coli Strains with Oxidizing Cytoplasmic Space

From: Expression of active human sialyltransferase ST6GalNAcI in Escherichia coli

Strain

Genotype

Source

DHB4 (parental)

Δ(ara-leu)7697 araD139 ΔlacX74 galE galK rpsL phoR

Δ(phoA)PvuII ΔmalF3 thi/F Δlac-pro lacIq

Boyd et al.1

SMG96

Δgor ΔtrxB ahpC*

Ref. [12]

FA113

gor 522.miniTn10 (TetR) ΔtrxB::KanR ahpC*

Ref. [11]

Origamiâ„¢2

gor 522:miniTn10 trxB::StrepR, TetR ahpC*

Novagen

MJF256.10

ΔgshA::KanRΔtrxB::CmR ahpC V164G

Ref. [12]

MJF277.2

ΔgshB::KanRΔtrxB ahpC*

Ref. [12]

MJF313.5

ΔgshA ΔtrxB::CmR ahpC E171 stop

Ref. [12]

DR611

ΔtrxA ΔtrxC trxB::KanR gor 522:Tn10 ahpC T104P/G141C

J. Beckwith

  1. 1. Boyd D, Manoil C, Beckwith J: Determinants of membrane protein topology. Proc Natl Acad Sci USA 1987, 84(23):8525-8529.