Expression of active human sialyltransferase ST6GalNAcI in Escherichia coli

Table 1 Plasmids used in this work

Plasmid	Protein expressed	Antibiotic Marker	Origin of replication	Source
pTrcST6	FLAG-ST6-His₈	AmpR	ColE1	This work
pCWinMBP-ST6	MBP-ST6	KanR	ColE1	This work
pBAD33	Empty vector	CmR	ACYC	Guzman et al.¹
pBADtig	Trigger Factor	CmR	ACYC	Ref. [17]
pBADΔssDsbA	Signal sequence-less DsbA (ΔssDsbA)	CmR	ACYC	Ref. [11]
pBADΔssDsbC	Signal sequence-less DsbC (ΔssDsbC)	CmR	ACYC	Ref. [11]
pAKJ	DnaK/DnaJ	CmR	ACYC	Perez-Perez et al.²
pAG	GroEL/GroES	CmR	ACYC	Perez-Perez et al.²
pAS	Signal sequence-less Skp (ΔssSkp)	CmR	ACYC	Ref. [17]

1. Guzman LM, Belin D, Carson MJ, Beckwith J: Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol 1995, 177(14):4121-4130.
2. Perez-Perez J, Martinez-Caja C, Barbero JL, Gutierrez J: DnaK/DnaJ supplementation improves the periplasmic production of human granulocyte-colony stimulating factor in Escherichia coli. Biochem Biophys Res Commun 1995, 210(2):524-529.

ISSN: 1475-2859