Table 1 Plasmids and oligonucleotide primers used in this study

Plasmids

   pHT304

Amp^rEm^r, E. coli-B. thuringiensis shuttle cloning vector, 6528 bp

[39]

   pGFPuv

Amp^r, plasmid vector carrying a variant of gfp, 3336 bp

CLONTECH Lab, Inc.

   pBMB3305

Amp^rEm^r, pHT304 derivative harbouring insecticidal gene cry3Aa with promoter P _cry3Aa , ~12.6 kb

Laboratory collection

   pMB172

Amp^r, pTYB12 derivative harbouring wlacD, 8867 bp

[20]

   pMB160

Amp^rEm^r, pHT304 derivative harbouring mba-gfp fusion gene, 8194 bp

This study

   pMB161

Amp^rEm^r, pHT304 derivative harbouring mbp-gfp fusion gene, 8667 bp

This study

   pMB162

Amp^rEm^r, pHT304 derivative harbouring mbg-gfp fusion gene, 8648 bp

This study

   pMB163

Amp^rEm^r, pHT304 derivative harbouring promoter P _cry3Aa , mbg and gfp, 9365 bp

This study

   pMB164

Amp^rEm^r, pHT304 derivative harbouring P _cry3Aa , mbgn and gfp, 8466 bp

This study

   pMB165

Amp^rErm^r, pHT304 derivative harbouring P _cry3Aa , mbgc and gfp, 9002 bp

This study

   pMB166

Amp^rEm^r, pHT304 derivative harbouring P _cry3Aa , lysM₁ and gfp, 8276 bp

This study

   pMB167

Amp^rEm^r, pHT304 derivative harbouring P _cry3Aa , lysM₂ and gfp, 8294 bp

This study

   pMB168

Amp^rEm^r, pHT304 derivative harbouring P _cry3Aa , (mbgn)₂ and gfp, 8777 bp

This study

   pMB169

Amp^rEm^r, pHT304 derivative harbouring P _cry3Aa , (mbgn)₃ and gfp, 9098 bp

This study

   pMB173

Amp^rEm^r, pHT304 derivative harbouring P _cry3Aa and wlacD, 8879 bp

This study

   pMB174

Amp^rEm^r, pHT304 derivative harbouring P _cry3Aa , (mbgn) ₂ and wlacD, 9521 bp

This study

Oligonucleotide primers^b

   BT101

5'-CGAGAATTC TGACAAGTGCGACACGTT-3'

   BT102

5'-TTCTTCTCCTTTACTCATACAGAAAATATGTTTACCG-3'

   BT103

5'-CGGTAAACATATTTTCTGTATGAGTAAAGGAGAAGAA-3'

   BT301

5'-CGTGAATTC CACTGTCAGTATAACACC-3'

   BT302

5'-TTCTTCTCCTTTACTCATCCTAACTAAATATGGCAG-3'

   BT303

5'-CTGCCATATTTAGTTAGGATGAGTAAAGGAGAAGAA-3'

   P1

5'-TTACCCGGG CTTCCCTTCTTTCACTTC-3'

   P2

5'-TTCTTCTCCTTTACTCATGCCCTTTTTCGTAATCGT-3'

   P3

5'-ACGATTACGAAAAAGGGCATGAGTAAAGGAGAAGAA-3'

   P4

5'-AAACTGCAG TTATTTGTAGAGCTCATCCATGC-3'

   P5

5'-ACGGGAATTC GGATTCAAAATAGCCCTG-3'

   P6

5'-CTGTCTAGA CGGATTCATTTTTCTTCC-3'

   P7

5'-CCAAGTTCTAGA ATGATTCAAATTGTAACGG-3'

   P8

5'-GCTAGATCT GCCCTTTTTCGTAATCGT-3'

   P9

5'-ACGAGATCT ATGAGTAAAGGAGAAGAA-3'

   P10

5'-TCTAGAGATCT GATGGATTCTACAGCTCG-3'

   P11

5'-CTGTCTAGA GTTAATGCTACACGTGCC-3'

   P12

5'-GCAAGATCT AACGATAAGTGCCTGACC-3'

   P13

5'-CGCTCTAGA TATGTACAGCCTGGTGAC-3'

   P14

5'-CGCGGATCC ATGATTCAAATTGTAACGG-3'

   P15

5'-CTGTCTAGA ATGCAACGTCGTGATTTC-3'

   P16

5'-AAACTGCAG TTATACCGTAAACCCTAAC-3'

   P17

5'-GCAAGATCT ATGCAACGTCGTGATTTC-3'