Plasmids or primers | Phenotypes or sequencesa | Source or reference |
---|---|---|
Plasmids | Â | Â |
   pHT304 | AmprEmr, E. coli-B. thuringiensis shuttle cloning vector, 6528 bp | [39] |
   pGFPuv | Ampr, plasmid vector carrying a variant of gfp, 3336 bp | CLONTECH Lab, Inc. |
   pBMB3305 | AmprEmr, pHT304 derivative harbouring insecticidal gene cry3Aa with promoter P cry3Aa , ~12.6 kb | Laboratory collection |
   pMB172 | Ampr, pTYB12 derivative harbouring wlacD, 8867 bp | [20] |
   pMB160 | AmprEmr, pHT304 derivative harbouring mba-gfp fusion gene, 8194 bp | This study |
   pMB161 | AmprEmr, pHT304 derivative harbouring mbp-gfp fusion gene, 8667 bp | This study |
   pMB162 | AmprEmr, pHT304 derivative harbouring mbg-gfp fusion gene, 8648 bp | This study |
   pMB163 | AmprEmr, pHT304 derivative harbouring promoter P cry3Aa , mbg and gfp, 9365 bp | This study |
   pMB164 | AmprEmr, pHT304 derivative harbouring P cry3Aa , mbgn and gfp, 8466 bp | This study |
   pMB165 | AmprErmr, pHT304 derivative harbouring P cry3Aa , mbgc and gfp, 9002 bp | This study |
   pMB166 | AmprEmr, pHT304 derivative harbouring P cry3Aa , lysM1 and gfp, 8276 bp | This study |
   pMB167 | AmprEmr, pHT304 derivative harbouring P cry3Aa , lysM2 and gfp, 8294 bp | This study |
   pMB168 | AmprEmr, pHT304 derivative harbouring P cry3Aa , (mbgn)2 and gfp, 8777 bp | This study |
   pMB169 | AmprEmr, pHT304 derivative harbouring P cry3Aa , (mbgn)3 and gfp, 9098 bp | This study |
   pMB173 | AmprEmr, pHT304 derivative harbouring P cry3Aa and wlacD, 8879 bp | This study |
   pMB174 | AmprEmr, pHT304 derivative harbouring P cry3Aa , (mbgn) 2 and wlacD, 9521 bp | This study |
Oligonucleotide primersb | Â | Â |
   BT101 | 5'-CGAGAATTC TGACAAGTGCGACACGTT-3' |  |
   BT102 | 5'-TTCTTCTCCTTTACTCATACAGAAAATATGTTTACCG-3' |  |
   BT103 | 5'-CGGTAAACATATTTTCTGTATGAGTAAAGGAGAAGAA-3' |  |
   BT301 | 5'-CGTGAATTC CACTGTCAGTATAACACC-3' |  |
   BT302 | 5'-TTCTTCTCCTTTACTCATCCTAACTAAATATGGCAG-3' |  |
   BT303 | 5'-CTGCCATATTTAGTTAGGATGAGTAAAGGAGAAGAA-3' |  |
   P1 | 5'-TTACCCGGG CTTCCCTTCTTTCACTTC-3' |  |
   P2 | 5'-TTCTTCTCCTTTACTCATGCCCTTTTTCGTAATCGT-3' |  |
   P3 | 5'-ACGATTACGAAAAAGGGCATGAGTAAAGGAGAAGAA-3' |  |
   P4 | 5'-AAACTGCAG TTATTTGTAGAGCTCATCCATGC-3' |  |
   P5 | 5'-ACGGGAATTC GGATTCAAAATAGCCCTG-3' |  |
   P6 | 5'-CTGTCTAGA CGGATTCATTTTTCTTCC-3' |  |
   P7 | 5'-CCAAGTTCTAGA ATGATTCAAATTGTAACGG-3' |  |
   P8 | 5'-GCTAGATCT GCCCTTTTTCGTAATCGT-3' |  |
   P9 | 5'-ACGAGATCT ATGAGTAAAGGAGAAGAA-3' |  |
   P10 | 5'-TCTAGAGATCT GATGGATTCTACAGCTCG-3' |  |
   P11 | 5'-CTGTCTAGA GTTAATGCTACACGTGCC-3' |  |
   P12 | 5'-GCAAGATCT AACGATAAGTGCCTGACC-3' |  |
   P13 | 5'-CGCTCTAGA TATGTACAGCCTGGTGAC-3' |  |
   P14 | 5'-CGCGGATCC ATGATTCAAATTGTAACGG-3' |  |
   P15 | 5'-CTGTCTAGA ATGCAACGTCGTGATTTC-3' |  |
   P16 | 5'-AAACTGCAG TTATACCGTAAACCCTAAC-3' |  |
   P17 | 5'-GCAAGATCT ATGCAACGTCGTGATTTC-3' |  |