Figure 8
Verification of surface display of WlacD in recombinant B. thuringiensis MB174 cells. (A) Western blot analysis of cell fractions prepared from MB174 cells expressing (Mbgn)2-wlacD and MB173 cells expressing cellular wlacD. WC, whole cell fractions; CW, cell-wall fractions; CC, cell cytoplasmic fraction. (B) Microscopic observation of MB173 and MB174 intact cells that were treated with anti-WlacD polyclonal antibody and followed with goat anti-mouse Cy3-conjugate antibody. (C) Measurement of whole-cell laccase activity using ABTS as the substrate. Each value and error bar represents the mean of three independent experiments and its standard deviation.