Table 1 In vitro 3-hydroxybutyral-CoA synthetase activity assay
Conditions | Concentration of 3HB (mM) at: | ||
|---|---|---|---|
0 h | 6 h | 12 h | |
E.coli enzyme extract, 3HB, CoA | 3.99 ± 0.06 | 3.86 ± 0.10 | 3.86 ± 0.06 |
E.coli enzyme extract, 3HB, ATP | 3.33 ± 0.02 | 3.41 ± 0.04 | 3.29 ± 0.05 |
E.coli enzyme extract, 3HB, ATP, CoA | 4.39 ± 0.03 | 2.02 ± 0.02 | 0.15 ± 0.10 |