Skip to main content

Table 1 In vitro 3-hydroxybutyral-CoA synthetase activity assay

From: Complete PHB mobilization in Escherichia coli enhances the stress tolerance: a potential biotechnological application

Conditions Concentration of 3HB (mM) at:
  0 h 6 h 12 h
E.coli enzyme extract, 3HB, CoA 3.99 ± 0.06 3.86 ± 0.10 3.86 ± 0.06
E.coli enzyme extract, 3HB, ATP 3.33 ± 0.02 3.41 ± 0.04 3.29 ± 0.05
E.coli enzyme extract, 3HB, ATP, CoA 4.39 ± 0.03 2.02 ± 0.02 0.15 ± 0.10