Figure 6

Supposed metabolic network between PHB biosynthesis and mobilization in recombinant E. coli DH5α (pQWQ2/pSCP-CAB). Dashed lines represent enzymatic steps engineered in recombinant E. coli. Solid lines represent enzymatic steps existed in wild type E. coli. A question mark denotes unidentified enzyme in E. coli. ① β-ketothiolase, PhaA ② NADPH-dependent acetoacetyl-CoA reductase, PhaB ③ PHB synthase, PhaC ④ PHB depolymerase, PhaZ ⑤ acyl-CoA synthetase or thioesterase ⑥ 3-hydroxyacyl-CoA dehydrogenase, 3-hydroxybutyryl-CoA epimerase, FadB ⑦ acetoacetyl-CoA transferase, AtoA, AtoD ⑧ acetyl-CoA acetyltransferase, AtoB.