Figure 5

Precursor feeding experiment with a clone from FL2 library containing a truncated flavonoid pathway (see text) on an approximately 500 Kb eYAC. Using natural precursors, a variety of flavonoids are produced including the expected flavanone and flavanol. From top to bottom: No precursor; cinnamic acid, yielding pinocembrin (5.26) and galangin (5.26); coumaric acid, yielding naringenin (3.84) and kaempferol (3.90); caffeic acid, yielding eriodictyol (3.30) and quercitin (3.33); and finally umbellic acid, yielding 5,7,2',4'-tetrahydroxy-flavanone (3.35) and morin (3.02).