Table 3 Candidate reactions whose disruption increases the lactate or succinate production flux predicted by FBA simulations.
Reaction disabled by gene deletion | Lactate production flux (mmol/gDW/h) | Growth rate (1/h) |
|---|---|---|
(Wild type) | 3.33 (1.00) | 9.54 × 10-2 (1.00) |
ADP + Pi + 4H [e] → ATP + H2O + H | 5.13 (1.54) | 8.50 × 10-2 (0.89) |
R5P + Xu5P ↔ S7P + GAP | 5.02 (1.51) | 9.06 × 10-2 (0.95) |
MAL ↔ FUM + H2O | 4.99 (1.50) | 8.97 × 10-2 (0.94) |
G6P + NADP → 6PGL + NADPH + H | 4.99 (1.50) | 9.06 × 10-2 (0.95) |
Reaction disabled by gene deletion | Succinate production flux (mmol/gDW/h) | Growth rate (1/h) |
(Wild type) | 1.05 (1.00) | 9.54 × 10-2 (1.00) |
NADH + PYR + H ↔ LAC + NAD | 2.24 (2.13) | 8.11 × 10-2 (0.85) |