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Figure 8 | Microbial Cell Factories

Figure 8

From: Robo-Lector – a novel platform for automated high-throughput cultivations in microtiter plates with high information content

Figure 8

Results of the method biomass-specific replication. (A) Growth of precultures with different initial biomass concentrations, from left to right (in triplicate): optical density at start 0.2, 0.1, 0.05, 0.025, 0.0125, 0.005; after 8.2 h the first cultivation was stopped and the automated method for replication (see also Figure 4) started. (B) Main culture: growth of 18 replicated cultures; the relative standard deviation of the scattered light after 7.8 h is ± 4%. BioLector conditions: 200 μL MDG mineral medium, 3 mm shaking diameter, 950 rpm shaking frequency, 37°C, strain E.coli BL21(DE3) pRhotHi-2-EcFbFP.

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Microbial Cell Factories

ISSN: 1475-2859

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