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Figure 7 | Microbial Cell Factories

Figure 7

From: Robo-Lector – a novel platform for automated high-throughput cultivations in microtiter plates with high information content

Figure 7

Effects of biomass-specific induction on E.coli BL21(DE3) pRhotHi-2-EcFbFP. (A) Growth of different inoculated cultures, induced at an induction criterion of 50 a.u. with 0.1 mM IPTG; cultures from left to right (in sixfold): optical density at start = 0.16 (green curves), 0.11 (red curves), 0.05 (black curves), 0.03 (blue curves). (B) Mean and standard deviation of normalized growth of 24 cultures from Figure 7A; the growth kinetics were normalized to have the same induction time as the first induced culture. (C) EcFbFP production of all induced cultures. (D) Mean and standard deviation of normalized product formation of 24 cultures from Figure 7C. BioLector conditions: 190 μL MDG mineral medium, 3 mm shaking diameter, 950 rpm shaking frequency, 37°C.

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Microbial Cell Factories

ISSN: 1475-2859

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