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Figure 5 | Microbial Cell Factories

Figure 5

From: Robo-Lector – a novel platform for automated high-throughput cultivations in microtiter plates with high information content

Figure 5

Influence of induction time and inducer concentration on EcFbFP production of E.coli BL21(DE3) pRhotHi-2-EcFbFP. 96 Induction experiments were conducted according to the profile shown in Figure 2B. Scattered light intensities of all cultures (A) and cultures induced with 0.05 mM IPTG at various points of time, as indicated by arrows (B). EcFbFP production of all cultures (C) and cultures induced with 0.05 mM IPTG (D). In Figure 5B and 5D also a reference cultivation (no induction) is shown; BioLector conditions: 190 μL MDG mineral medium, 3 mm shaking diameter, 950 rpm shaking frequency, 37°C.

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Microbial Cell Factories

ISSN: 1475-2859

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