Figure 3
Principle of method biomass-specific induction. (A) Simplified process flow chart; shaded boxes indicate necessary user input; gray arrows indicate interaction of pipetting robot and BioLector; MTP: microtiter plate; parameters scind: scattered light at induction (induction criterion), v: volume of inducer added per well, ip: induction phase, T: cultivation temperature, n: shaking frequency. (B) Schematic principle of the established procedure for biomass-specific induction; throughout the complete cultivation, subsequent induction phases are conducted for the whole microtiter plate; please see Materials and Methods section for more details.