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Table 3 Enzyme activities (U/(mg total protein)): dehydrogenases

From: Arabinose and xylose fermentation by recombinant Saccharomyces cerevisiae expressing a fungal pentose utilization pathway

Enzyme

Substratea

Strain

LAD

L-ARABITOL

XYLITOL

 
 

0.77 ± 0.10

0.58 ± 0.07

TMB3659

ALXa, LAD, XDH

0.94 ± 0.04

(0.45 ± 0.02)

28.54 ± 4.91

(21.13 ± 5.57)

TMB3664

XDH

0.72 ± 0.01

(0.25 ± 0.00)

7.20 ± 0.56

(7.88 ± 0.88)

TMB3665

  1. NAD+ was used as a cofactor for all reactions. a. Standard substrate concentration: 330 mM. Values between parentheses are referred to assays performed with substrate concentration of 100 mM.