Table 1 Plasmids and strains used in this study
Plasmid | Features | Reference |
|---|---|---|
pUCsLAD1 | pUC57 + synthetic sequence based on T. reesei Lad1p | Genscript Corp, Piscataway, USA |
pUCsALX1 | pUC57 + synthetic sequence based on A. monospora Alx1p | Genscript Corp, Piscataway, USA |
YIpOB5 | ADH1p-XYL1(K270R)-ADH1t, PGK1p-XYL2-PGK1t, URA3 | [37] |
YIpOB7 | TDH3p-ADH1t, PGK1p-XYL2-PGK1t, URA3 | Bengtsson O, Bettiga M, Garcia-Sanchez R, Gorwa-Grauslund MF: Differential behaviour of two commonly used promoters in xylose utilizing recombinant Saccharomyces cerevisiae; unpublished. |
YIpOB9 | TDH3p-XYL1(K270R)-ADH1t, PGK1p-XYL2-PGK1t, URA3 | This work |
p425GPD | TDH3 p-CYC1 t, LEU2 | [44] |
p425GPD_LAD1 | TDH3 p-lad1(T. reesei, cDNA)- CYC1 t, LEU2 | This work |
p425GPD_ALX1 | TDH3 p-ALX1(A. monospora)- CYC1 t, LEU2 | This work |
p425GPD_sLAD1 | TDH3 p-sLAD1(synthetic)- CYC1 t, LEU2 | This work |
p425GPD_sALX1 | TDH3 p-sALX1(synthetic)- CYC1 t, LEU2 | This work |
p425GPD_sLAD1_sALX1 | TDH3 p-sLAD1(synthetic)- CYC1 t; TDH3 p-sALX1(synthetic)- CYC1 t, LEU2 | This work |
Strain | Genotype | Reference |
T. reesei Rut-C30 | Wild type | NRRL 11460 |
A. monospora PYCC4390T | Wild type | Kindly provided by Prof. Isabel Spencer-Martins |
CEN.PK 113-16B | MAT a, leu2-3 112 | [47] |
TMB3043 | CEN.PK 2-1C, MAT a, leu2-3 112, ura3-52, Δgre3, his3::HIS3 PGK1p-XKS1-PGK1t, TAL1::PGK1p-TAL1-PGK1t, TKL1::PGK1p-TKL1-PGK1t, RKI1::PGK1p-RKI1-PGK1t, RPE1::PGK1p-RPE1-PGK1t | [36] |
TMB3656 | CEN.PK 113-16B, p425GPD_ALX1 | This work |
TMB3657 | CEN.PK 113-16B, p425GPD_LAD1 | This work |
TMB3658 | CEN.PK 113-16B, p425GPD_sALX1 | This work |
TMB3659 | CEN.PK 113-16B, p425GPD_sLAD1 | This work |
TMB3660 | CEN.PK 113-16B, p425GPD | This work |
TMB3662 | TMB3043, YIpOB9 | This work |
TMB3664 | TMB3662, p425GPD_sLAD1_sALX1 | This work |
TMB3665 | TMB3662, p425GPD | This work |