Regioselective biooxidation of (+)-valencene by recombinant E. coli expressing CYP109B1 from Bacillus subtilis in a two-liquid-phase system

Table 3 Yields of nootkatol (2 and 3) and (+)-nootkatone (4) in biphasic systems with 10% dodecane^a

Cww [g l^-1]	c_valencene [mg l^-1]	Conversion [%]^b	Yield [mg g^-1 cdw]	Yield [mg l^-1]
70	409	15.1	3.35	61.6
70	613	13.2	4.40	80.9
70	818	11.8	5.22	96.0
140	409	21.8	2.42	89.1
280	409	23.0	1.28	94.2
140	818	14.1	3.14	118.5

^aConversion of (+)-valencene (1) was carried out with recombinant E. coli expressing PdR, Pdx and CYP109B1 in 20 ml CV2 buffer with 2% DMSO for 8 h at 30°C and extracted with ethyl acetate. Cell wet weight (cww) was as indicated; 70 g cww correspond to 18.4 g cell dry weight (cdw). Values are shown as mean of two experiments.
^bPercental amount of (+)-valencene (1) that was converted to nootkatol (2 and 3) and (+)-nootkatone (4)

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