Figure 4

Product distributions of (+)-valencene (1) conversions in biphasic systems. Conversion of 2 mM (+)-valencene (1) was carried out with recombinant E. coli expressing PdR, Pdx and CYP109B1 in biphasic systems consisting of 2 ml CV2 buffer, 2% DMSO and organic solvents (v/v) as indicated for 8 h at 30°C and extracted with ethyl acetate. Values represent mean ± standard deviation, n ≥ 3. "Products" sum up the percental amounts of the total product of the two nootkatol isomers (2 and 3) and (+)-nootkatone (4), "byproducts" combine the percental amounts of the total product of the compounds with RT 12.1, 12.3 and 13.3 min, respectively.