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Figure 2 | Microbial Cell Factories

Figure 2

From: Regioselective biooxidation of (+)-valencene by recombinant E. coli expressing CYP109B1 from Bacillus subtilis in a two-liquid-phase system

Figure 2

GC diagrams of extracts of (+)-valencene (1) conversions. Conversion of 2 mM (+)-valencene (1) was carried out with recombinant E. coli expressing PdR, Pdx and either P450MoxA or CYP109B1 in 2 ml aqueous CV2 buffer with 2% DMSO for 8 h at 30°C and extracted with ethyl acetate. Numbered peaks represent cis-nootkatol (2), trans-nootkatol (3), (+)-nootkatone (4) and overoxidation products (5, 6 and 7). * Conversion products of (+)-valencene (1) that could not be identified. ** Non-specific peaks that were also present in the negative control.

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