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Table 4 Conditions of the bioreactor adaptation phase under optimal DoE conditions.

From: Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime

Time (min)

T (°C)

pH

DO (%)

0

30

6

30

15

28

6.4

45

30

25

6.8

60

45

23

7.2

75

60

21.5

7.6

90

75

21.5

7.6

90

  1. The input factors, T (°C), pH and DO (%) were adjusted every 15 min over the course of 1 h via an ADI1030 controller. Once optimal DoE conditions (21.5°C, pH 7.6, 90% DO) were reached, a further 15 min stabilisation period was applied prior to fed-batch induction.