From: Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime
T(°C)
pH
DO(%)
20
7.5
60
7.7
80
27
8
50
28
90
6
23.6
7.25
27.5
6.7
6.5
6.3
21.5
7.6
40