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Figure 3 | Microbial Cell Factories

Figure 3

From: Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime

Figure 3

Examination of the influence of culture vessel on GFP yield. Shake flask cultures were set-up with a working volume of 20% total vessel volume, the M24 with 60% and the bioreactor with 43%. Vented cultures had a 0.2 μm integral filter as a closure. Non-vented cultures had a foil closure. Culture conditions were 30°C, pH 6 and either 250 rpm in the shake flasks, or DO 30% in the M24 and the 7 L bioreactor. The model predicts a specific yield of 219 ng mL-1 OD595-1. Total yields for each condition are given.

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