Molecular cloning and expression of a novel trehalose synthase gene from Enterobacter hormaechei

Table 2 Effects of metal ions and chemicals on the activity of recombinant TreS

Reagent	Relative activity (%)	Reagent	Relative activity (%)
None	100*	Cu²⁺	52 ± 4.7
Hg²⁺	57 ± 2.2	Na⁺	90 ± 2.4
Mg²⁺	98 ± 1.3	NH₄ ⁺	101 ± 4.3
Ca²⁺	103 ± 7.9	SDS	10 ± 1.1
Zn²⁺	59 ± 1.3	EDTA	98 ± 5.8
Fe²⁺	92 ± 5.9	β-ME	0
Mn²⁺	111 ± 10.7

* The enzyme activity of TreS without the addition of ions or reagents was taken to be 100%.

ISSN: 1475-2859