Figure 2From: PimT, an amino acid exporter controls polyene production via secretion of the quorum sensing pimaricin-inducer PI-factor in Streptomyces natalensisGene replacement of pimT. A) Predicted restriction enzyme polymorphism caused by gene replacement. The Afl III restriction pattern before and after replacement is shown. The probe is indicated by thick lines. B, Bam HI; F, Afl III; L, Apa LI; N, Nru I; P, PvuI. B) Southern hybridization of the Afl III digested chromosomal DNA of the wild type (lane 1), and ΔpimT (lane 2) strains.Back to article page