Components of the E. coli envelope are affected by and can react to protein over-production in the cytoplasm

Table 2 Intensity changes of selected spots identified from 2-DE gels. Numbers refer to Figure 3.

n°	*name*	*Anova (p)* ^a	*function*	*ratio BCL*	*ratio GFP*	*references*
3	LamB	0.00017	maltoporin	3.8	4.7	[35, 50, 51]
4	OmpF	0.014	outer membrane protein F	3.0	2.0	[35, 36, 53]
9	OmpA	0.008	outer membrane protein A	1.7	2.4	[26, 35, 49]
6	TolC	0.076	outer membrane TolC	2.0	2.0	[46, 52]
5	EF-Tu	0.015	Elongation factor Tu	1.3	0.9
1	GroEL	n.a.	HSP 60	1.1	0.9
7	DLDH	n.a.	Dihydrolipoyl dehydrogenase	1.0	0.9
2	ATPB	>>	ATP synthase subunit beta
8	TolB	>>	Protein tolB
10	GFP	>>	over-expressed GFP
11	RS6	>>	30S ribosomal protein S6
12	OmpX	>>	outer membrane protein X
13	SSB	>>	single-stranded DNA binding protein
14	DPS	>>	DNA protection during starvation protein
15	IbpA	>>	small heat shock protein IbpA

Ratios were calculated from the normalized spot intensities averaged for gel replicates and finally expressed as BCL- or GFP-over-Control ratios. The first block contains membrane proteins affected by over-expression with statistical significance.
^a Probability score calculated by the program taking in consideration all previous parameters. "n.a." means volume was calculated from the sum of all spot intensities belonging to the same protein (as determined by ESI-MS spot identification for multiplets), due to excessive protein amount in gels or poor focusing. ">>" indicate value was much above threshold, no ratio is reported although protein presence was determined by mass analysis.

ISSN: 1475-2859