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Table 2 Intensity changes of selected spots identified from 2-DE gels. Numbers refer to Figure 3.

From: Components of the E. coli envelope are affected by and can react to protein over-production in the cytoplasm

name Anova (p) a function ratio BCL ratio GFP references
3 LamB 0.00017 maltoporin 3.8 4.7 [35, 50, 51]
4 OmpF 0.014 outer membrane protein F 3.0 2.0 [35, 36, 53]
9 OmpA 0.008 outer membrane protein A 1.7 2.4 [26, 35, 49]
6 TolC 0.076 outer membrane TolC 2.0 2.0 [46, 52]
5 EF-Tu 0.015 Elongation factor Tu 1.3 0.9  
1 GroEL n.a. HSP 60 1.1 0.9  
7 DLDH n.a. Dihydrolipoyl dehydrogenase 1.0 0.9  
2 ATPB >> ATP synthase subunit beta    
8 TolB >> Protein tolB    
10 GFP >> over-expressed GFP    
11 RS6 >> 30S ribosomal protein S6    
12 OmpX >> outer membrane protein X    
13 SSB >> single-stranded DNA binding protein    
14 DPS >> DNA protection during starvation protein    
15 IbpA >> small heat shock protein IbpA    
  1. Ratios were calculated from the normalized spot intensities averaged for gel replicates and finally expressed as BCL- or GFP-over-Control ratios. The first block contains membrane proteins affected by over-expression with statistical significance.
  2. a Probability score calculated by the program taking in consideration all previous parameters. "n.a." means volume was calculated from the sum of all spot intensities belonging to the same protein (as determined by ESI-MS spot identification for multiplets), due to excessive protein amount in gels or poor focusing. ">>" indicate value was much above threshold, no ratio is reported although protein presence was determined by mass analysis.