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Figure 9 | Microbial Cell Factories

Figure 9

From: Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter plates

Figure 9

Characterization of promoters. MOX and FMD promoter regulation in Hansenula polymorpha on glucose and glycerol growth medium monitored via GFP fluorescence intensities and parallel measurement of scattered light intensities; Hansenula polymorpha wt, RB11-pC10-Mox-GFP and RB11-pC10-FMD-GFP in YPD (10 g/L glucose) and YPG (20 g/L glycerol) medium; measured in 96 well MTP, with 200 μL filling volume, at 37°C temperature, 995 rpm shaking frequency and 3 mm shaking diameter, scattered light (ex: 620 nm/em: -, Gain: 20), GFP (ex: 485 nm/em: 520 nm, Gain: 10).

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