Figure 9From: Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter platesCharacterization of promoters. MOX and FMD promoter regulation in Hansenula polymorpha on glucose and glycerol growth medium monitored via GFP fluorescence intensities and parallel measurement of scattered light intensities; Hansenula polymorpha wt, RB11-pC10-Mox-GFP and RB11-pC10-FMD-GFP in YPD (10 g/L glucose) and YPG (20 g/L glycerol) medium; measured in 96 well MTP, with 200 μL filling volume, at 37°C temperature, 995 rpm shaking frequency and 3 mm shaking diameter, scattered light (ex: 620 nm/em: -, Gain: 20), GFP (ex: 485 nm/em: 520 nm, Gain: 10).Back to article page