Figure 7
Clone Screening – comparison of growth and GFP protein expression of 90 different Hansenula polymorpha clones. (A) growth via scattered light intensities (B) protein expression via GFP fluorescence intensities; (C) volumetric productivity (PV) – calculated as GFP formation rate without consideration of setup time of the equipment, the best clones are depicted by given the well number in the diagram; 45 clones of Hansenula polymorpha RB11-pC10-Mox-GFP and 45 clones of Hansenula polymorpha RB11-pC10-FMD-GFP in buffered YNB-G medium; measured in 96 well MTP, with 200 μL filling volume, at 37°C temperature, 995 rpm shaking frequency and 3 mm shaking diameter, scattered light (ex: 620 nm/em: -, Gain: 20), GFP (ex: 485 nm/em: 520 nm, Gain: 10).