Figure 6

Comparison of different media – growth and protein expression of a flavin mononucleotide (FMN)-based fluorescent protein ( FbFP ) in E. coli. E. coli BL21-Pet 28A ytvAC62A culture in LB, TB and WR media; measured in 96 well MTP, with 200 μL filling volume, at 30°C temperature, 995 rpm shaking frequency and 3 mm shaking diameter, induction at 7.3 h with 0.5 mM IPTG, scattered light (ex: 620 nm/em: -, Gain: 20), FbFP (ex: 460 nm/em: 520 nm, Gain: 10).