Figure 4

Influence of pH conditions on growth – buffered/unbuffered medium monitored with scattered light intensities and NADH fluorescence intensities. Hansenula polymorpha RB11-pC10-FMD-GFP culture in buffered YNB medium with 10 g/L glycerol and 100 mM phosphate and unbuffered YNB medium with 10 g/L glycerol without phosphate; measured in 96 well MTP, with 200 μL filling volume, at 30°C temperature, 995 rpm shaking frequency and 3 mm shaking diameter, scattered light (ex: 620 nm/em: -, Gain: 20), NADH (ex: 340 nm/em: 460 nm, Gain: 20).