Figure 10

Influence of filling volume. Growth and pH monitoring of E. coli cultures with filling volumes from 200 μl to 290 μL; E. coli BL21 culture in TB medium, measured in 96 well MTP, with 200, 230, 260 and 290 μL filling volume, at 30°C temperature, 995 rpm shaking frequency and 3 mm shaking diameter, scattered light (ex: 620 nm/em: -, Gain: 20), HPTS (ex: 410 nm and 460 nm/em: 510 nm, Gain: 10), calibration parameters for the Boltzmann equation: I_{R, min}= 0.00, I_{R, max}= 3.00, dpH = 0.50, pH₀ = 7.20.