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Table 2 Expression plasmids for T. maritima 6PGDH with or without the tags

From: Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration

Plasmid

Modular organization

Molecular mass

pET-trx-wt6pgdh

Trx-WT6PGDH

66, 634 Da

pET-ci-wt6pgdh

CBM-intein-WT6PGDH

91, 187 Da

pET-ci-co6pgdh

CBM-intein-CO6PGDH

91, 187 Da

pET-co6pgdh-his

CO6PGDH-(His)6

53, 975 Da

pET-co6pgdh

CO6PGDH

53, 152 Da

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Microbial Cell Factories

ISSN: 1475-2859

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