Figure 3
SDS-PAGE analysis of the expression and purification of T. maritima 6PGDH from plasmid pET-ci-co6pgdh in the E. coli BL21(DE3) (A) and E. coli BL21(DE3)-RIL (B). M, protein marker; BI, before induction; T, total cellular proteins; S, soluble proteins; P, purified protein. Cells were grown at 37°C until A600 of 0.6. The induction condition was 20 μM IPTG at 20°C for 6 h.