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Table 2 Secreted proteins of P. pastoris

From: Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris

PIPA ID

Predicted function

theoretical pI/MW [kDa]

Predicted N-glycosylation sites

Predicted localization

PIPA00211

Covalently-bound cell wall protein of unknown function

5.01/45.73

1

secreted

PIPA00246

hypothetical fungal hexokinase

5.98/24.92

1

no SP

PIPA00436

Cell wall protein related to glucanases

4.83/36.07

0

secreted

PIPA00545

Cell wall protein related to glucanases

4.33/45.02

2

secreted

PIPA00748

O-glycosylated protein required for cell wall stability

4.22/31.86

1

secreted

PIPA00934

SCP-domain family protein, unknown function, extracellular

5.55/31.72

0

secreted

PIPA00956

60S ribosomal protein L18A

9.92/21.82

1

no SP

PIPA01008

GAS1; Beta-1,3-glucanosyltransferase

3.98/57.20

4

secreted

PIPA01010

GAS1; Beta-1,3-glucanosyltransferase

3.99/58.37

5

secreted

PIPA01223

potential cell wall glucanase

4.34/49.39

0

secreted

PIPA01958

Endo-beta-1,3-glucanase

4.03/33.76

1

secreted

PIPA02332

no similarity found

6.01/23.64

2

no SP

PIPA02510

Glyceraldehyde-3-phosphate dehydrogenase

6.24/35.74

1

no clear SP

PIPA02524

glucan 1,3-beta-glucosidase similar to S. cerevisiae EXG1 (YLR300W)

4.51/46.22

1

secreted

PIPA02544

aldehyde dehydrogenase, Adh2p [S. cerevisiae]

6.00/36.86

0

no SP

PIPA03955

endo-1,3-beta-glucanase [P. stipitis CBS 6054], Dse4p [S. cerevisiae]

4.70/109.45

5

secreted

PIPA04722

Cell wall protein with similarity to glucanases

5.18/32.95

0

secreted

PIPA05357

no similarity found

4.25/66.46

1

no SP, 2 TM

PIPA05673

YLR286Cp-like protein [S. cerevisiae], endochitinase

4.05/71.87

1

no clear SP

PIPA05771

Chitin deacetylase, Cda2p [S. cerevisiae]

5.25/34.66

2

secreted, lower probability

  1. List of identified secreted proteins, with theoretical pI and theoretical MW, and information on the predicted localization (SP = signal peptide, TM = transmembrane domain).