Heme and menaquinone induced electron transport in lactic acid bacteria

Table 3 Heme and menaquinone-stimulated growth of lactic acid bacteria.

Species		+K₂		+Heme		+heme +K₂
		OD600	pH	OD600	pH	OD600	pH
Enterococcus faecalis	B145	5.27	5.62	5.56	5.82	7.30	5.82
Lactobacillus plantarum	WCFS1	8.31	3.88	7.88	3.92	10.2	4.36
Lactobacillus rhamnosus	B637	1.55	4.15	1.51	4.17	3.59	4.01
Lactococcus garviae	DSM 6783	2.84	4.61	2.98	4.77	3.57	4.80
Lactobacillus brevis	B306	8.43	4.15	9.05	4.14	10.5	4.17
Lactobacillus paralimentarius ¹	B1357	1.17	4.51	2.21	4.28	1.98	4.37
Streptococcus entericus ¹	B2339	3.96	4.42	5.1	5.48	5.32	5.49
		g/l		g/l		g/l
Streptococcus agalactiae ²	NEM316	1.4	4.8	1.4	4.8	2.1	5.6

¹Should be considered heme-stimulated ²see reference [19]
Lactic acid bacteria were grown aerobically in the presence of 2 μg/ml heme (+heme) and/or 20 μg/ml vitamin K₂ (+K₂), or with equivalent volumes of ethanol or 0.05 M NaOH as control. A clear increase in biomass yield is visible after a 48 hour incubation period when both heme and vitamin K₂ are added to the growth medium. The experiments were performed at least in four-fold and the data shown represents averages.

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