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Figure 4 | Microbial Cell Factories

Figure 4

From: Factors affecting plasmid production in Escherichia coli from a resource allocation standpoint

Figure 4

Effects of Pyk flux and acetate by-production on maximum plasmid yield and other key fluxes. The model was run for different constrained combinations of r acetate and r Pyk in Equations (8) and (9), with α = 250 in Equation (5) and r transhydrogenase = 0 in every case. Specific (r acetate , r Pyk ) zones are drawn in each panel for JM101 (wild-type) and PB25 (pykF pykA) based on experimentally determined ranges. On all plots, JM101 and PB25 operating regions are shown by a white zone and a thick black line, respectively. (A) Maximum plasmid yield. Plasmid-producing results fall into two regions, Planes A and B, whose characteristics are discussed in the text. (B) Bla marker production. (C) HMP:glycolysis flux ratio. (D) Ppc flux as % glucose uptake. (E) Mez flux as % glucose uptake. (F) Mdh flux as % glucose uptake, where the white line is the locus of solutions where Mdh flux = 0.

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