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Figure 2 | Microbial Cell Factories

Figure 2

From: Factors affecting plasmid production in Escherichia coli from a resource allocation standpoint

Figure 2

Optimal carbon flux distributions for maximum plasmid production in E. coli. In all three distributions,r transhydrogenase was constrained to zero, while r acetate and r Pyk were not constrained. Distributions vary by their assigned value of α in Equation (5) and/or whether or not a limit was imposed on Bla production. Top:α = 0. Middle:α = 250; unlimited Bla production. Bottom:α = 250; Bla production limited to 20% of total protein (Equation (7), f = 0.20). For simplicity, the network in Fig. 1 has been condensed and fluxes (mmol/g/h) have been expressed as % of glucose uptake, except r Bla (expressed as % of total protein) and r plasmid (expressed as yield in mg/g). The production of lactate and succinate have also been omitted, as these fluxes were nil for all distributions.

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