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Table 2 Estimated optimum free fluxes (mmol/100 mmol Glucose) and the associated 90% confidence interval.

From: OpenFLUX: efficient modelling software for 13C-based metabolic flux analysis

  

Wild-type

Mutant

Flux parameter

 

Optimum

90% CI

Optimum

90% CI

Glucose-6P dehydrogenase

A

46.8

[46.3, 47.3]

56.2

[55.9, 56.5]

 

B

46.7

[46.3, 47.1]

56.3

[56.1, 56.5]

 

C

 

[46.2, 47.3]

 

[56.0, 56.6]

Pyruvate carboxylase

A

71.5

[67.6, 75.7]

62.6

[60.0, 64.8]

 

B

74.9

[70.9, 79.3]

63.6

[61.8, 65.6]

 

C

 

[70.4, 79.5]

 

[61.2, 65.7]

Glucose-6P isomerase (rev)

A

1.3

[1.2, 1.3]

2.4

[2.3, 2.4]

 

B

1.27

[1.26, 1.30]

2.37

[2.33, 2.39]

 

C

 

[1.24, 1.31]

 

[2.32, 2.41]

Transketolase 1 (rev)

A

0

[2.1, 2.5]

0

[0, 0]

 

B

2.21

[2.03, 2.35]

0

[0, 0]

 

C

 

[1.93, 2.49]

 

[0, 0]

Transaldolase (rev)

A

2.2

[2, 2.3]

4.5

[4.3, 4.6]

 

B

2.16

[2.06, 2.24]

4.50

[4.40, 4.62]

 

C

 

[2.00, 2.33]

 

[4.31, 4.69]

Transketolase 2 (rev)

A

1

[0.7, 1.3]

1.6

[1.4, 1.7]

 

B

1.02

[0.84, 1.21]

1.50

[1.44, 1.57]

 

C

 

[0.73, 1.41]

 

[1.41, 1.61]

  1. A. Original work [39]; B. Gradient-based search; C. Monte Carlo. B's and C's optimum values are derived from the best optimum solution found by OpenFLUX. The unit for fluxes and confidence intervals is mmol/100 mmol glucose.